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草坪草种的品种真实性鉴定是评定草种质量的重要指标。依据《国际种子检验规程》 ,采用等电聚焦电泳技术对适于西北地区的几种冷季型草坪草种进行了品种真实性鉴定研究 ,确定了两性电解质的pH值范围、酶提取液浓度及最佳取材时间 ,证明等电聚焦电泳方法是鉴别草坪草品种的有效测定技术。
The authenticity identification of the turf grass species is an important index for evaluating the quality of the grass species. According to the “International Seed Testing Regulations”, isoelectric focusing electrophoresis technique was applied to authenticity identification of several cold-season turfgrasses suitable for the northwestern China. The pH range of the ampholytes, the concentration of the enzyme extract, The best time to draw, to prove isoelectric focusing electrophoresis method is to identify the effective determination of turfgrass varieties of technology.