论文部分内容阅读
目的探讨云南省彝族乳腺癌遗传易感性与CYP1A1基因MspⅠ位点多态性的关系。方法采用聚合酶链反应-限制性片段长度多态性PCR-RFLP技术,对云南省肿瘤医院51例彝族乳腺癌患者及60例健康女性人群的CYP1A1基因3′端限制性内切酶MspI位点基因多态性进行分析。结果乳腺癌组C等位基因分布频率(51.0%)明显高于对照组(33.3%),差异均有统计学意义(均P<0.05)。3种基因型分布频率与对照组相比,差异有统计学意义(均P<0.05)。TC、CC基因型患乳腺癌的危险度分析OR(95%CI)分别是TT基因型的1.19和1.95倍。结论云南省彝族乳腺癌发病率可能与CYP1A1基因型有关,基因突变增加了患乳腺癌的风险。
Objective To investigate the genetic susceptibility of Yi ethnicity in Yunnan province and its association with the MspⅠ polymorphism of CYP1A1 gene. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the MspI site of CYP1A1 gene in 51 cases of Yi nationality breast cancer patients and 60 healthy females in Yunnan Cancer Hospital. Genetic polymorphisms were analyzed. Results The frequencies of C allele in breast cancer group (51.0%) were significantly higher than those in control group (33.3%) (all P <0.05). The distribution frequency of the three genotypes was significantly different from that of the control group (all P <0.05). The risk of breast cancer in genotype TC and CC OR (95% CI) were 1.19 and 1.95 times that of TT genotype respectively. Conclusions The incidence of breast cancer in Yi nationality in Yunnan Province may be related to the CYP1A1 genotype. Mutation of the gene may increase the risk of breast cancer.