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观察荆芥挥发油对内毒素(LPS)中毒模型小鼠的保护作用,并采用GC-MS对荆芥挥发油进行化学成分测定。雄性C57BL/6J小鼠按体重分层随机分为空白对照组、模型对照组、地塞米松组(5 mg·kg-1)、荆芥挥发油0.226 g·kg-1及0.452g·kg-1剂量组。除地塞米松组实验当日腹腔注射给药一次外,其余各组小鼠连续灌胃给药5 d,1次/d。各组末次给药30min后,除空白组小鼠外,其余各组小鼠腹腔注射LPS(15 mg·kg-1)制备内毒素中毒模型。造模12 h后,小鼠取血分离血清,采用ELISA及液相蛋白芯片技术测定炎症因子IL-18,IL-1β,IL-5,TNF-α,单核细胞趋化蛋白-1(MCP-1),巨噬细胞炎性蛋白(MIP-1β),巨噬细胞集落刺激因子(M-CSF)及粒细胞-巨噬细胞集落刺激因子(GM-CSF)的水平;剖取肺脏、脾脏、胸腺称重,计算脏器指数;取小鼠全血进行白细胞(WBC)、血小板(PLT)计数;同时进行各组小鼠肺组织病理组织学检查。GC-MS检测结果显示薄荷酮、胡薄荷酮的相对质量分数分别为46.67%,33.92%,二者质量分数占总挥发油的80.59%。荆芥挥发油预防给药,0.452,0.226 g·kg-1剂量均能显著降低模型小鼠血清IL-1β,IL-5,TNF-α,MCP-1,MIP-1β,M-CSF水平(P<0.01或P<0.05),荆芥挥发油0.452 g·kg-1剂量亦降低血清IL-18,GM-CSF水平(P<0.01或P<0.05);荆芥挥发油0.226 g·kg-1剂量能使肺组织内嗜中性粒细胞浸润减少,显示出良好抗炎效应。但荆芥挥发油预防给药,对模型小鼠白细胞计数的升高、血小板计数的减少及脾脏指数、肺指数的升高、胸腺指数的降低无明显干预作用。结果表明,荆芥挥发油预防给药对内毒素(LPS)中毒模型小鼠有一定保护作用,作用发挥主要与抑制各类炎性细胞因子的释放,减轻炎症反应有关。
To observe the protective effect of volatile oil of Nepeta on mice with LPS poisoning and the chemical constituents of volatile oil of Nepeta were determined by GC-MS. Male C57BL / 6J mice were randomly divided into blank control group, model control group, dexamethasone group (5 mg · kg -1), Nepeta volatile oil 0.226 g · kg -1 and 0.452 g · kg -1 Dose group. Except for dexamethasone group, intraperitoneal injection was given on the day of experiment, and other mice in each group were given gavage for 5 days and once daily. The rats in each group were injected with LPS (15 mg · kg-1) intraperitoneally to prepare the model of endotoxin poisoning. After 12 h of modeling, the mice were sacrificed and the serum was separated. The serum levels of IL-18, IL-1β, IL-5, TNF-α and monocyte chemoattractant protein-1 -1), MIP-1β, M-CSF and GM-CSF. The lung and spleen , The thymus was weighed and the organ index was calculated. The white blood cells (WBC) and platelet count (PLT) were taken from whole blood of mice, and the histopathological examination of the lungs was also performed. The GC-MS results showed that the relative mass fractions of menthone and pentmentone were 46.67% and 33.92%, respectively, and the mass fractions of them accounted for 80.59% of the total volatile oil. Nepeta volatile oil could prevent the administration of 0.452,0.226 g · kg-1 dose, which could significantly reduce the level of IL-1β, IL-5, TNF-α, MCP-1, MIP-1β and M-CSF (P <0.01 or P <0.05). The dose of 0.452 g · kg-1 of Nepeta volatile oil also decreased the level of IL-18 and GM-CSF in serum (P <0.01 or P < The lung tissue neutrophil infiltration decreased, showing a good anti-inflammatory effect. Nepeta volatile oil, however, was not prevented by the administration of volatile oil. It had no significant effect on the increase of leucocyte count, reduction of platelet count, spleen index, pulmonary index and thymus index in model mice. The results showed that the Nepeta volatile oil preventive administration of LPS poisoning model mice have a protective effect, play a major role in the inhibition of the release of various inflammatory cytokines, reduce the inflammatory response.