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AIM:To investigate the association of the NQO1 (C609T)polymorphism with susceptibility to esophageal squamouscell carcinoma (ESCC) and gastric cardiac adenocarcinoma(GCA) in North China.METHODS:The NQO1 C609T genotypes were determinedby polymerase chain reaction-restriction fragment lengthpolymorphism (PCR-RFLP) analysis in 317 cancer patients(193 ESCC and 124 GCA) and 165 unrelated healthy controls.RESULTS:The NQO1 C609T C/C,C/T and T/T genotypefrequency among healthy controls was 31.5%,52.1% and16.4% respectively.The NQO1 T/T genotype frequencyamong ESCC patients (25.9%) was significantly higher thanthat among healthy controls (X~2=4.79,P=0.028).The NQO1T/T genotype significantly increased the risk for developingESCC compared with the oombination of C/C and C/T genotypes,with an age,sex and smoking status adjusted odds ratio (OR)of 1.78 (1.04-2.98).This increased susceptibility waspronounced in ESCC patients with family histories of uppergastrointestinal cancers (UGIC) (adjusted OR=2.20,95%CI=1.18-3.98).Similarly,the susceptibility of the NQO1 T/Tgenotype to GCA development was also observed amongpatients with family histories of UGIC,with an adjusted oddsratio of 2.55 (95% CI=1.21-5.23),whereas no differencein NQO1 genotype distribution was shown among patientswithout family histories of UGIC.CONCLUSION:Determination of the NQO1 C609T genotypemay be used as a stratification marker to predicate theindividuals at high risk for developing ESCC and GCA in NorthChina.
AIM: To investigate the association of the NQO1 (C609T) polymorphism with susceptibility to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in North China. METHODS: The NQO1 C609T genotypes were determined by polymerase chain reaction- restriction fragment length polymorphism RFLP) analysis in 317 cancer patients (193 ESCC and 124 GCA) and 165 unrelated healthy controls. RESULTS: The NQO1 C609T C / C, C / T and T / T genotype frequency of healthy controls was 31.5%, 52.1% and 16.4 The NQO1 T / T genotypes significantly increased the risk for developing ESCC compared with the oombination (X = 2 = 4.79, P = 0.028) of C / C and C / T genotypes, with an age, sex and smoking status adjusted odds ratio (OR) of 1.78 (1.04-2.98) .This increased susceptibility waspronounced in ESCC patients with family histories of upper gastrointestinal cancers (UGIC) (adjusted OR = 2 .20, 95% CI = 1.18-3.98). Similarly, the susceptibility of the NQO1 T / T genotype to GCA development was also observed amongpatients with family histories of UGIC, with an adjusted odds ratio of 2.55 (95% CI = 1.21-5.23) , and no difference in NQO1 genotype distribution was shown in patients with out family histories of UGIC.CONCLUSION: Determination of the NQO1 C609T genotypemay be used as a stratification marker to predicate the individuals at high risk for developing ESCC and GCA in NorthChina.