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目的探讨肿瘤热休克蛋白70(HSP70-PCs)联合含CpG寡脱氧核苷酸(CpG-ODN)对荷瘤模型的治疗作用及可能机制。方法纯化肝癌细胞中的HSP70-PCs,SDS-PAGE及Western-blot技术对产物进行鉴定。建立小鼠肝癌皮下移植瘤模型,分别运用HSP70-PCs、CpG-ODN和HSP70-PCs联合CpG-ODN对其进行治疗,观察治疗作用。ELISA进行免疫后细胞因子分析。结果二者联合可产生强大的抗肿瘤作用,肿瘤体积、质量、抑瘤率、肿瘤转移率及生存期限与两药单独使用比较差异有统计学意义;二者联合可以提高荷瘤小鼠血清IFN-γ及CXCL-10水平,与二者单独使用比较差异有统计学意义。结论联合使用HSP70-PCs和CpG-ODN能显著提高机体免疫功能,对荷瘤小鼠有显著治疗作用,可能与诱导Th1型细胞因子产生有关。
Objective To investigate the therapeutic effect and possible mechanism of tumor heat shock protein 70 (HSP70-PCs) combined with CpG-ODN on tumor-bearing model. Methods Purified liver cancer cells HSP70-PCs, SDS-PAGE and Western-blot technology to identify the product. The mouse model of subcutaneous xenograft of hepatocellular carcinoma was established and treated with HSP70-PCs, CpG-ODN and HSP70-PCs in combination with CpG-ODN to observe the therapeutic effect. ELISA for post-immunization cytokine analysis. Results The combination of the two could produce a powerful antitumor effect. The tumor volume, quality, tumor inhibition rate, tumor metastasis rate and survival period were significantly different from those of the two drugs alone. The combination of the two could increase the serum IFN -γ and CXCL-10 levels, with the difference between the two alone was statistically significant. Conclusions The combined use of HSP70-PCs and CpG-ODN can significantly improve immune function and have a significant therapeutic effect on tumor-bearing mice, which may be related to the induction of Th1-type cytokines.