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目的探讨益生菌对实验性结肠炎大鼠肠道炎症、菌群及黏膜肿瘤坏死因子(TNF)-α表达的影响。方法建立2,4,6-三硝基苯磺酸(TNBS)实验性结肠炎大鼠模型,30只Wistar大鼠均分为正常对照组(NC组)、模型对照组(UC组)和益生菌治疗组(PC组)。PC组大鼠采用双歧三联活菌悬液(2．2×10~9 CFU/只)灌胃治疗,1次/d,共4周,光镜下观察肠黏膜炎症并评分,检测各组大鼠部分肠道菌群、肠黏膜中TNF-α、白细胞介素(IL)-6表达及血浆内毒素水平,分析其变化。结果PC组大鼠结肠炎症评分较UC组明显改善(7．94±0．85比10．25±1．36,P＜0．05),但未恢复至NC组水平(7．94±0．85比4．35±0．88,P＜0．01)。PC组肠道内乳酸杆菌和双歧杆菌较UC组明显增加,大肠杆菌和真菌则减少(P＜0．05)。PC组血浆内毒素、TNF-α水平较UC组降低[内毒素：(93．33±21．22)pg/ml比(1 21．25±39．07)pg/ml；TNF-α：67．51±14．63比85．99±18．17,P值均＜0．05],但高于NC组[内毒索：(93．33±21．22)pg/ml比(35．20±15．12)pg/ml；TNF-α：67．51±14．63比43．28±19．98,P值均＜0．01]。与UC组比较,PC组IL-6水平有所降低,但差异无统计学意义(155．22±34．01比184．09±29．11,P＞0．05),且高于NC组(155．22±34．01比108．73±37．35,P＜0．01)。结论双歧三联活菌可调整肠道菌群紊乱,减轻大鼠结肠炎症,改善菌群紊乱、减少内毒素吸收及局部促炎性细胞因子的分泌。
Objective To investigate the effect of probiotics on intestinal inflammation, flora and mucosal tumor necrosis factor (TNF) -α expression in experimental colitis rats. Methods A rat model of experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) was established. Thirty Wistar rats were divided into normal control group (NC group), model control group (UC group) and probiotics Bacteria treatment group (PC group). Rats in PC group were treated with bifidobacterium triple viable suspension (2.2 × 10 ~ 9 CFU / g) once a day for 4 weeks. Intestinal mucosal inflammation was observed under light microscope and scored. The changes of TNF-α, IL-6 and endotoxin levels in some intestinal flora and intestinal mucosa of rats were analyzed. Results The colitis score in PC group was significantly improved compared with UC group (7.94 ± 0.85 vs 10.25 ± 1.36, P <0.05), but not to NC group (7.94 ± 0 .85 vs 4.35 ± 0.88, P <0.01). Lactobacillus group and Bifidobacterium group in PC group were significantly increased compared with UC group, while Escherichia coli and fungi decreased (P <0.05). The levels of plasma endotoxin and TNF-α in PC group were lower than those in UC group [endotoxin: (93.33 ± 21.22) pg / ml (121.25 ± 39.07) pg / ml; .51 ± 14.63 vs 85.99 ± 18.17, all P <0.05], but higher than that in NC group (93.33 ± 21.22 pg / ml). 20 ± 15.12) pg / ml; TNF-α: 67.51 ± 14.63 vs 43.28 ± 19.98, both P <0.01]. The level of IL-6 in PC group was lower than that in UC group, but the difference was not statistically significant (155.22 ± 34.01 vs 184.09 ± 29.11, P> 0.05), which was higher than that in NC group (155.22 ± 34.01 vs. 108.73 ± 37.35, P <0.01). Conclusion Bifidobacterium triple viable bacteria can regulate intestinal flora disorders, reduce colonic inflammation in rats, improve flora, reduce endotoxin absorption and local proinflammatory cytokine secretion.