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生物炭是一种可以改良土壤、增强作物产量和提升作物品质的新型农林废弃物再利用材料。本研究通过振荡方式制备生物炭浸提液,利用水培系统培养水稻幼苗,以20%PEG6000、300 mmol/L和500 mmol/L甘露醇模拟干旱胁迫,研究生物炭表面水溶活性分子对水稻幼苗抗旱性的影响。研究结果发现:生物炭浸提液可有效缓解干旱胁迫造成的水稻种子萌发和幼苗生长抑制,缓解叶绿素含量、鲜重及存活率的降低,同时可以降低体内的活性氧积累等。实时定量RT-PCR检测表明生物炭浸提液促进干旱胁迫响应标志基因的表达量。研究结果说明生物炭浸提液可以提高干旱胁迫下水稻幼苗的抗氧化能力,进一步提高水稻幼苗对干旱胁迫的耐受性。
Biochar is a new type of agricultural and forestry waste recycling material that can improve soil, enhance crop yield and improve crop quality. In this study, biochar extracts were prepared by shaking culture, and the rice seedlings were cultured by hydroponic culture system. The effects of biochar surface water-soluble active molecules on rice seedlings were studied by simulating drought stress with 20% PEG6000, 300 mmol / L and 500 mmol / L mannitol Drought Resistance. The results showed that the biochar extract can effectively alleviate the seed germination and seedling growth inhibition caused by drought stress, alleviate the decrease of chlorophyll content, fresh weight and survival rate, and reduce the accumulation of reactive oxygen species in vivo. Real-time quantitative RT-PCR showed that the biochar extract enhanced the expression of drought-stress response marker genes. The results showed that the biochar extract can improve the antioxidant capacity of rice seedlings under drought stress and further improve the tolerance of rice seedlings to drought stress.