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本实验用间接致癌物二亚硝基哌嗪(dinitrosopiperazine DNP)处理人胚鼻咽上皮细胞,以人胚肾上皮为对照,观察不同组织内DNP诱发的姊妹染色单体交换(Sistar Chromatid exchanges SCEs)的频率。结果表明:人胚鼻咽上皮可以活化DNP,表明诱发的SCE濒率增加,具量效关系;相同剂量DNP处理后,鼻咽上皮内诱发的SCEs频率明显高于肾脏上皮细胞,表现器官特异性的SCE反应,证实了人胚鼻咽上皮细胞有活化DNP酶系的性能。
In this experiment, human embryonic nasopharyngeal epithelial cells were treated with indirect carcinogen dinitrosopiperazine (DNP), and human embryonic kidney epithelium was used as control to observe the sister-chromatid exchange (SCE) induced by DNP in different tissues. Frequency of. The results showed that DNP could be activated in human embryonic nasopharyngeal epithelium, suggesting that the induced SCE delirium rate increased with a dose-effect relationship. After the same dose of DNP treatment, the frequency of SCEs induced in nasopharyngeal epithelium was significantly higher than that of renal epithelial cells, showing organ-specificity. The SCE response confirmed the performance of human embryonic nasopharyngeal epithelial cells with an activated DNP enzyme system.