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平均体重150克的虹鳟,饲喂添加或不添加泛酸的半纯制日粮。16周后,饲喂泛酸缺乏日粮鱼饲料摄入量下降,28周时,添加泛酸鱼体重>600克,而泛酸缺乏鱼体重为425克。泛酸缺乏鱼在25周时出现厌食和精神不振,28周时开始死亡。鳃组织的组织检查发现,缺乏泛酸鱼鳃瓣溶合,进一步证实泛酸缺乏。间隔4 周,采集鱼的鳃、肝、心脏和红细胞通过RIA测定每组鱼样本游离和总泛酸含量,结合泛酸的浓度通过差异计算,除鳃外,各日粮处理组间无差异。饲喂泛酸缺乏鱼日粮4周以后,鳃和心脏中游离泛酸浓度较饲喂添加鱼显著降低,饲喂16周后泛酸缺乏日粮鱼的肝中游离泛酸浓度与添加鱼间差异显著,在8周和以后饲喂泛酸缺乏鱼红细胞游离泛酸浓度与始值差异显著。应用红细胞中游离泛酸浓度,检测鱼泛酸缺乏是非常有价值的。
Rainbow trout weighing an average of 150 grams were fed semi-pure diets with or without pantothenic acid. After 16 weeks, feed intake of pantothenic acid-deficient diet decreased, with pantothenic acid body weight> 600 g at week 28 and pantothenic acid-deficient fish weighing 425 g. Pantothenic acid deficiency Anorexia and sluggishness at 25 weeks and death at 28 weeks. Tissue examination of the gill tissue revealed a lack of pantothenic acid gill flap fusion, further evidence of pantothenic acid deficiency. Four weeks apart, gill, liver, heart and erythrocyte were collected from fish for determination of free and total pantothenic acid content in each fish sample by RIA. The concentration of pantothenic acid was calculated by difference. Except gill, there was no difference between the two groups. After 4 weeks of feeding pantothenic acid deficiency diet, the concentration of free pantothenic acid in the gills and heart was significantly lower than that of the fish fed prawn supplementation. After 16 weeks of feeding, there was significant difference between the concentration of free pantothenate There was significant difference between the initial concentration and the free pantothenic acid concentration of fish fed erythrocytes after 8 weeks and beyond. The use of free pantothenate concentration in red blood cells, the detection of pantothenic acid deficiency is very valuable.