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[Objective] To study the best synthetic endosperm combination of Atractylodes macrocephala Koidz.seeds and to provide the theoretical basis for development and large-scale application of A.macrocephala synthetic seeds.[Method] Factors to affect germination rate and seedling conversion rate,such as encapsulated propagule and synthetic endosperm components were studied with germfree tube plantlet system of A.macrocephala.[Result] The germination rate and seedling conversion rate of synthetic seeds could reach 100% and 92% with auxilizry buds,2.5% sodium alginate + 2% CaCl2 as synthetic endosperm encapsulation,and 0.5 mg/L IAA and 30 g/L sucrose as synthetic endosperm components.[Conclusion] The technique of rapid multiplication of synthetic seeds on the basis of tissue culture of A.macrocephala has definite superiority.
[Objective] To study the best synthetic endosperm combination of Atractylodes macrocephala Koidz. Seeds and to provide the theoretical basis for development and large-scale application of A. macrocephala synthetic seeds. [Method] Factors to affect germination rate and seedling conversion rate, such as encapsulated propagule and synthetic endosperm components were studied with germfree tube plantlet system of A. macrocephala. [Result] The germination rate and seedling conversion rate of synthetic seeds could reach 100% and 92% with auxilizry buds, 2.5% sodium alginate + 2% CaCl2 as synthetic endosperm encapsulation, and 0.5 mg / L IAA and 30 g / L sucrose as synthetic endosperm components. [Conclusion] The technique of rapid multiplication of synthetic seeds on the basis of tissue culture of A. macrocephala has definite superiority.