生物钟是生物长期适应地球昼夜循环变化而演化的内在自持的计时机制。生物钟对分子、生化、细胞、生理和行为等基本生命过程起到重要的调节作用。生物钟紊乱会导致机体失调而引发各类疾病。斑马鱼作为一种重要的脊椎动物模型在研究生物钟方面有其独特的特点与优势。现主要综述利用转基因技术、突变体分析和转录组手段等解析斑马鱼生物钟调节机制的最新进展。 The biological clock is the internal self-sustaining timing mechanism for the biological evolution to adapt to the circadian changes of the earth’s long-term. The biological clock plays an important regulatory role in the basic life processes of molecules, biochemistry, cells, physiology and behavior. Biological clock disorders lead to disorders and trigger various diseases. As an important vertebrate model, zebrafish has its unique characteristics and advantages in studying the circadian clock. This review summarizes the recent advances in the regulation of the clock regulation of zebrafish using transgenic techniques, mutant analysis and transcriptomic approaches.