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目的 :探讨母体慢性铅暴露对子代鼠在体海马齿状回长时程增强 (long- term potentiation,L TP)的影响。方法 :饮蒸馏水和 0 .2 %醋酸铅溶液两组母鼠的子代鼠对应分为对照组和母体期铅暴露组 ,应用细胞外微电极记录单脉冲刺激穿通路纤维在海马齿状回诱发的群体锋电位 (population spides,PS) ,观察并比较两组于高频刺激(high- frequency stim ulation,HFS)前后的 PS幅值变化。结果 :HFS前 ,基线记录的两组 PS平均幅值及峰潜伏期无显著差异 ;HFS后 ,母体期铅暴露组 L TP发生率虽与对照组无显著差异 ,但其 L TP增幅减小 ,并出现了短时增强转为抑制及 L TD型反应。统计显示母体铅暴露组 HFS后 PS振幅的平均增强率显著低于对照组 ,对照组平均增强至基线值的 (138.2± 10 .4) % ,母体期铅暴露组为基线值的 (10 8.8± 2 0 .7) %。结论 :母体慢性铅暴露可损害子代鼠海马齿状回 L TP的在体诱导和维持 ,且以维持过程受损为主。
Objective: To investigate the effects of chronic lead exposure on long-term potentiation (LTP) in offspring rats. Methods: The offspring of distilled water and 0.2% lead acetate solution were divided into control group and lead exposure group. The extracellular microelectrode recording single pulse was used to stimulate the dentate gyrus induced in the hippocampus Population spides (PS). The changes of PS amplitude before and after high frequency stimulus (HFS) were observed and compared. Results: There was no significant difference in baseline mean peak amplitude and peak latency between the two groups before HFS. After HFS, the incidence of L TP in the lead exposure group was no significant difference compared with that of the control group Appeared short-term enhancement into inhibition and L TD type reaction. Statistics showed that the average enhancement rate of PS amplitude after HFS in maternal lead exposure group was significantly lower than that in control group (138.2 ± 10.4%) on average, and maternal lead exposure group was baseline (10 8.8 ± 2 0 .7)%. Conclusion: Chronic lead exposure may impair the in vivo induction and maintenance of LTP in the hippocampal dentate gyrus of the offspring, and mainly focus on maintaining the process.