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目的 提取一种成年鸡脑源性的神经生长抑制因子—— collapsin- 1并测定其功能。方法 鸡脑膜粗提物通过数个离子交换柱和麦胚凝集素亲和柱 ,再经 SDS- PAGE电泳纯化 ,用背根神经节 (DRG)培养物检测其致萎缩活性。结果 成年鸡脑膜提取物具有导致背根神经节生长冠萎缩的活性 ;随纯度的增加 ,活性也增加。10 0 kd的蛋白质与致萎缩活性紧密相关。结论 collapsin- 1为一种成年鸡脑源性神经生长抑制因子。
Objective To extract a brain-derived neurotrophic factor, collapsin-1, and determine its function. Methods The chicken cerebellum crude extract was passed through several ion exchange columns and wheat germ agglutinin affinity column and then purified by SDS-PAGE electrophoresis. The atrophy induced by dorsal root ganglion (DRG) culture was detected. Results The adult chicken brain extract had the activity of causing crown atrophy in dorsal root ganglion. The activity increased with the increase of purity. The protein of 10 kd is closely related to the activity of atrophy. Conclusion collapsin-1 is a brain-derived neuro-growth inhibitory factor in adult chicken.