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龙华清肝冲剂是具有清热利湿、清肝利胆的民族药,但本品质量标准过于简单。标准中只有定性鉴别,无定量指标,不能有效控制产品质量。本文采用反相高效液相色谱法对龙华清肝颗粒中的栀子苷含量进行测定,提高质量标准,结果准确,分离度好,操作简便。 1 仪器与试药 1.1 仪器LC-10A型高效液相色谱仪,SPD-10A检测器,LC-10A色谱工作站。 1.2 试药龙华清肝冲剂及缺栀子的阴性样品由某制药厂提供,栀子苷对照品由中国药品物制品定所提供。乙腈为色谱纯,
Longhua Qinggan granule is a national medicine with heat, dampness, and clearing of liver and gallbladder, but the quality standard of this product is too simple. Only the qualitative identification in the standard, no quantitative indicators, can not effectively control the product quality. This article uses RP-HPLC to determine the content of geniposide in Longhua Qinggan granules, improve the quality standards, the results are accurate, the degree of separation is good, and the operation is simple. 1 Instruments and reagents 1.1 Instrument LC-10A high performance liquid chromatography, SPD-10A detector, LC-10A chromatography workstation. 1.2 The negative samples of the Longhua Qinggan granules and the lack of wolfberry fruit were provided by a pharmaceutical factory, and the reference substance of jasminoidin was provided by the China National Pharmaceutical Products Co., Ltd. Acetonitrile is chromatographically pure,