副溶血性弧菌是我国沿海地区常见的食物中毒病原菌,主要存在于近海岸的海水、海水沉积物及鱼虾、贝类等海产品中[1-2]。人们食用受该菌污染的食品后,容易引起急性胃肠炎而导致腹泻、呕吐及肠痉挛等,严重的患者还会出现脱水、休克昏迷,甚至死亡。本文应用环介导等温扩增技术(LAMP)技术检测副溶血性弧菌的不耐热溶血毒素基因(tlh),同时以GB/T 4789.7—2008[1]中的 Vibrio parahaemolyticus is a common pathogen of food poisoning in the coastal areas of China. It mainly exists in seawater, seawater sediment and fish and shrimp, shellfish and other marine products near the coast [1-2]. People eat food contaminated by the bacteria, easily lead to acute gastroenteritis and cause diarrhea, vomiting and intestinal cramps, severe cases of dehydration, shock coma and even death. In this paper, the heat-labile toxin gene (tlh) of Vibrio parahaemolyticus was detected by ring-mediated isothermal amplification (LAMP) technique. At the same time,