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目的 :了解草酸钙晶体表面结合蛋白质在结石形成过程中的作用。方法 :用草酸钙过饱和结晶法制备正常人和草酸钙结石患者尿草酸钙晶体表面结合物质 ( CSBS) ,经 DEAE- SepharoseCL- 6B柱层析分离蛋白质和葡胺聚糖 ,用 SDS-聚丙烯酰胺凝胶电泳 ( SDS- PAGE)测定蛋白质组成和分子量 ,用氨基酸自动分析仪测定蛋白质的氨基酸组成。结果 :正常人 CSBS中主要含分子量为310 0 0的尿凝血酶原激活肽片段 1( UPTF1)和分子量为 670 0 0的白蛋白。结石患者 CSBS以白蛋白为主 ,其 UPTF1含量低于正常人。结论 :正常人和结石患者晶体表面结合蛋白质组成的差异可能与结石形成有关。
Objective: To understand the role of calcium oxalate crystal surface binding proteins in the formation of stones. Methods: Calcium oxalate crystal surface bound substance (CSBS) was prepared from normal and calcium oxalate stones by supersaturation with calcium oxalate. Proteins and glycosaminoglycans were separated by column chromatography on DEAE-Sepharose CL-6B. SDS- Protein composition and molecular weight were determined by gel electrophoresis (SDS-PAGE), and the amino acid composition of the protein was determined by an automatic amino acid analyzer. Results: Normal human CSBS mainly contained the prothrombin activator peptide fragment 1 (UPTF1) with a molecular weight of 310,000 and albumin with a molecular weight of 670,000. Stones in patients with albumin-based CSBS, the UPTF1 levels lower than normal. Conclusion: The differences of the crystal composition of the surface bound proteins in normal and stone patients may be related to the formation of stones.