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目的研究大鼠臂丛神经主要分支在神经干中的分布规律。方法用1,1′-双十八烷-3,3,3′,3′-四甲基吲哚羰花青-高氯酸盐(1,1′-diodadecyl-3,3,3′,3′-tetramethylindocarbocyanine perehlofate,Dil)作臂丛神经远端主要分支的逆行神经示踪,观察其纤维在近端神经干不同平面内的分布范围。结果DiI在神经干内的扩散速度:2 d组为:(8.819±0.32)mm/d、4d组为:(8.482±0.262)mm/d、6d组为:(8.062±0.108)mm/d。对于最低安全平面,桡神经中肱三头肌支位置最高,正中神经分支中桡侧屈腕肌、旋前圆肌支与主干融合的位置最高,而尺神经的分支最低安全平面均较低。结论DiI对神经束标记具有轴突特异性,可以用于活体神经束路的研究。DiI在活体臂丛神经中顺行、逆行扩散速度相同。正中神经、尺神经作为部分动力神经源移位于肌皮神经,在入肌点平面切取均是安全的。
Objective To study the distribution of the main branches of the brachial plexus in the neural stem. Methods 1,1'-dioctadecyl-3,3,3 ', 3'-tetramethylindocarbocyanine-perchlorate (1,1'-diodadecyl-3,3,3' 3'-tetramethylindocarbocyanine perehlofate, Dil) was used as a retrograde tracer for the distal branches of the brachial plexus to observe the distribution of the fibers in different planes of the proximal trunk. Results The diffusion rate of DiI in the neural stem was (8.819 ± 0.32) mm / d in 2 d, (8.482 ± 0.262) mm / d in 4d and (8.062 ± 0.108) mm / d in 6d. For the lowest level of safety, the position of the triceps branch in the radial nerve was the highest, and the position of the radial wrist flexor carina in the median nerve branch was the highest, while the branch of the pronator had the highest fusion with the trunk. The lowest safety level of the ulnar nerve branch was lower. Conclusion DiI has axon specificity for nerve bundle labeling and can be used for the study of living neural pathway. DiI in living brachial plexus in parallel, retrograde diffusion rate of the same. The median nerve, ulnar nerve, as part of the motor nerve source in the musculocutaneous nerve cut into the muscle plane plane are safe.