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目的研究菊花水煎液促进长双歧杆菌的体外增殖与活性的作用,初步探讨它作为一种双歧因子的条件。方法以菌液A600值、pH、活菌计数及发酵牛乳的凝乳时间、pH、滴定酸度等为指标,研究菊花水煎液对长双歧杆菌生长的影响,并用K-B琼脂法初步研究菊花水煎液对几种常见肠道菌群的抑/促菌作用。结果在5%添加菊花水煎液的培养基中长双歧杆菌的菌体浓度(A600值及活菌计数)均明显高于空白对照,菌液pH也均低于空白对照;长双歧杆菌发酵含有菊花水煎液的牛奶后,凝乳时间缩短,乳液的滴定酸度高于空白对照,pH也相应降低。菊花水煎液对几种常见肠道致病菌有较强的抑菌作用。结论菊花水煎液能促进长双歧杆菌的体外增殖与活性,且具有一定的选择性;菊花有可能成为一种良好的双歧因子或益生元制剂的候选物质。
Objective To study the effect of chrysanthemum water decoction on the proliferation and activity of Bifidobacterium longum in vitro, and to explore its condition as a bifidogenic factor. Methods The effects of decoction of chrysanthemum on the growth of Bifidobacterium longum were studied with A600 value of bacterial liquid, pH, viable counts, curd time of fermented milk, pH and titration acidity. A preliminary study on chrysanthemum water with KB agar method was conducted. Decoction inhibits/promotes bacteria in several common intestinal flora. Results The bacterial concentration (A600 value and viable count) of Bifidobacterium longum in medium supplemented with chrysanthemum decoction in 5% was significantly higher than that of the blank control. The pH of the broth was also lower than that of the blank control; Bifidobacterium longum After fermenting the milk containing chrysanthemum decoction, the curd time was shortened, the titration acidity of the emulsion was higher than that of the blank control, and the pH was also reduced accordingly. Chrysanthemum decoction has a strong antibacterial effect on several common intestinal pathogens. Conclusion Chrysanthemum decoction can promote the proliferation and activity of Bifidobacterium longum in vitro, and has certain selectivity. Chrysanthemum may become a good candidate for bifidus factor or prebiotic preparation.