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水稻(Oryza sativa)是重要的粮食作物,其花器官的正常起始及形态建成直接影响水稻的产量。为了深入分析水稻小花发育的调控机理,从已构建的水稻EMS诱变突变体库中筛选获得了一个花器官异常发育的突变体apl(abnormal palea and lodicules)。与野生型相比,apl突变体小花的内稃膨大,浆片伸长或转换成稃状结构,雄蕊数目减少,表明APL基因可能参与调控水稻内稃、浆片和雄蕊等多轮花器官属性的建成。遗传学分析表明,该突变体性状受1个隐性单基因控制。通过图位克隆,将APL基因初步定位于1号染色体上。该工作为深入研究APL基因在水稻花器官形态建成中的作用机制奠定了基础。
Oryza sativa is an important food crop. The normal initiation and morphogenesis of floral organs directly affects the yield of rice. In order to further analyze the regulatory mechanism of floret development in rice, a mutant apl (abnormal palea and lodicules) with abnormal floral organ development was screened from the mutagenized mutants of rice EMS. Compared with the wild type, apl mutant florets inflated the infrasound, elongated or converted into a squash-like structure, the number of stamens decreased, indicating APL gene may be involved in regulation of rice inflorescence, pulp and stamens and other round of flower organ properties Built. Genetic analysis showed that the trait was controlled by a recessive single gene. The APL gene was initially located on chromosome 1 by map-based cloning. This work lays the foundation for further study on the mechanism of APL gene in the formation of floral organ of rice.