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目的研究三邻甲苯磷酸酯(Tri-ortho-cresyl phosphate,TOCP)对Neuro-2a(N2a)细胞生长分化的影响,探索TOCP诱导迟发性神经病(OPIDN)发生的机制。方法培养N2a细胞,利用CCK8试剂盒检测TOCP抑制细胞增殖的浓度范围,然后分别以0、2.5、5、10和20μmol/L浓度的TOCP染毒N2a细胞,观察TOCP对视黄酸(Retinoic acid,RA)诱导N2a细胞分化的影响。在此基础上,利用TOCP染毒分化的N2a细胞,观察TOCP对N2a细胞轴突形态的影响。结果TOCP染毒引起未分化的N2a细胞分化能力显著减弱,轴突长度明显缩短;而分化的N2a细胞暴露于TOCP后,则出现明显的轴突损伤,且随着TOCP染毒剂量的增加,轴突损伤逐渐加重。结论 TOCP影响了N2a细胞轴突的生长分化,可能与其诱导OPIDN发病机制有关。
Objective To investigate the effect of Tri-ortho-cresyl phosphate (TOCP) on the growth and differentiation of Neuro-2a (N2a) cells and to explore the mechanism of TOCP-induced delayed neuropathy (OPIDN). Methods N2a cells were cultured and the concentration of TOCP was determined by CCK8 kit. Then N2a cells were exposed to TOCP of 0, 2.5, 5, 10 and 20 μmol / L, respectively. The effect of TOCP on retinoic acid (Retinoic acid, RA) on the differentiation of N2a cells. On this basis, TOCP-treated N2a cells were used to observe the effects of TOCP on the morphology of axon in N2a cells. Results TOCP induced a significant decrease in the differentiation ability of undifferentiated N2a cells and significantly shortened the length of axons. However, axonal damage was observed in differentiated N2a cells exposed to TOCP. With the increase of TOCP dose, Sudden damage gradually increased. Conclusion TOCP affects the axonal growth and differentiation of N2a cells, which may be related to the pathogenesis of OPIDN.