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[目的]建立鉴定广佛手黄龙病病原的有效方法,为无毒苗木的选育及病害防治提供早期诊断依据。[方法]在广佛手主要种植区进行调查,了解黄龙病的发病状况;根据柑桔黄龙病病原的DNA序列设计特异性的引物,对样品的DNA进行聚合酶链式反应(PCR)扩增。[结果]广东肇庆地区的广佛手出现类似柑桔黄龙病的症状,PCR检测结果表明,在表现发病症状的广佛手样品中有403 bp的特异性DNA片段出现,而健康的样品中则没有扩增出特征带,表明广佛手的感病症状是由黄龙病病原引起的。[结论]PCR技术是广佛手黄龙病病原鉴定的有效方法之一。
[Objective] The research aimed to establish an effective method for identifying the pathogen of Huanglongbing disease in Guangfa handicrafts and provide early diagnosis basis for breeding and disease prevention of non-toxic seedling. [Method] The prevalence of Huanglongbing was investigated in the main growing areas of Guangfandan. The specific primers were designed according to the DNA sequence of the pathogen of citrus Huanglongshan, and the samples were amplified by polymerase chain reaction (PCR). [Results] The results showed that there were 403 bp specific DNA fragments in Guang-berg samples showing symptoms, while there was no expansion in healthy samples Increased feature band, indicating that the symptoms of Guang-berg hands are caused by the pathogen of the Huanglong disease. [Conclusion] PCR technique is one of the effective ways to identify the pathogen of Guanglong hand Huanglong disease.