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背景 为了深入了解红系分化过程中Rh复合体的形成过 程,我们研究了改良的双相液体培养系统,在该培养系统中可产生Rh30和Rh相关糖蛋白,特别是Rh50。研究设计和方法 将新鲜外周血中单核细胞先在一种培养基中培养7天,该培养基含有一种从膀胱癌细胞株(5637)中提取的条件培养基。然后收集不贴壁细胞置入含有2U/ml促红素(引起红系分化)的第二种培养基中培养。用流式细胞仪观测第二阶段培养过程中(16天)Rh30和Rh50的表达。结果4天后D+细胞出现,8天后增加到70％。第12天后,90％的细胞表现D+,并持续到培养结束。
Background In order to gain insight into the formation of Rh complexes during erythroid differentiation, we investigated a modified biphasic liquid culture system in which Rh30 and Rh-related glycoproteins, especially Rh50, are produced. Study Design and Methods Monocytes from fresh peripheral blood were first cultured for 7 days in a medium containing a conditioned medium extracted from a bladder cancer cell line (5637). The non-adherent cells were then collected and cultured in a second medium containing 2U / ml erythro-erythroid (causing erythroid differentiation). Flow cytometry was used to observe Rh30 and Rh50 expression during the second phase of culture (16 days). Results D + cells appeared after 4 days and increased to 70% after 8 days. After day 12, 90% of the cells showed D + and continued until the end of the culture.