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采用比色法测定闹羊花素 Ⅲ (简称R Ⅲ )对菜粉蝶 5龄幼虫血淋巴和中肠酯酶的活性 ,并分析了酯酶同工酶聚丙烯酰胺凝胶电泳 (PAGE)图谱。活体试验结果表明 ,R Ⅲ以每虫 5 μg定量饲喂或以 2 0mg/L浸渍叶碟后饲喂试虫 ,能显著降低血淋巴和中肠酯酶比活力 ,以每虫 1～ 3μg处理或 5mg/L浸叶饲喂处理时 ,对酶活性的影响取决于处理剂量和时间。PAGE表明 ,R Ⅲ以每虫 5 μg处理 ,酯酶酶谱发生明显变化 ,主酶带明显抑制
The activities of esterase in hemolymph and midgut of 5th instar larvae of Pieris rapae were determined by colorimetric method and the polyacrylamide gel electrophoresis (PAGE) pattern of esomerase was analyzed. The results of in vivo tests showed that when R Ⅲ was fed in a dose of 5 μg per insect or fed on a leaf dish at 20 mg / L, the test insects were fed, and the esterase specific activity in the hemolymph and midgut was significantly reduced to 1 ~ 3 μg per insect Or 5 mg / L dipping leaves, the effect on enzyme activity depends on the dose and the time of treatment. PAGE showed that R Ⅲ was treated with 5 μg / insect, the esterase spectrum changed significantly, and the main enzyme band was significantly inhibited