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就应用强酸性阳离子交换树脂（Ｂ）及强碱性阴离子交换树脂（Ｇ）分离提纯发酵液中Ｌ－谷氨酰胺（Ｌ－Ｇｌｎ）的工艺条件进行了研究，通过单因子及正交试验确定离子交换分离法分离Ｌ－Ｇｌｎ的较佳条件为：强酸性阳离子交换树脂分离Ｌ－Ｇｌｎ的上柱液ｐＨ为３．５，洗脱剂氨水的浓度为０．０３ｍｏｌ／Ｌ，洗脱速度为树脂体积的０．６５％；强碱性阴离子交换树脂分离Ｌ－Ｇｌｎ的上柱液的ｐＨ为８．０，洗脱剂盐酸的浓度为０．０５ｍｏｌ／Ｌ，洗脱速度为树脂体积的１．１５％；使用上述条件提取Ｌ－Ｇｌｎ的得率为３０％左右。
The conditions for the separation and purification of L-glutamine (L-Gln) from the fermentation broth using strong acid cation exchange resin (B) and strong basic anion exchange resin (G) were studied by single factor and orthogonal test The optimum conditions for the separation of L-Gln by ion-exchange separation are as follows: the pH of the upper column for the separation of L-Gln from the strongly acidic cation exchange resin is 3.5, the concentration of the eluent ammonia is 0.03 mol / L, the elution rate is 0.65% by volume of the resin; the pH of the supernatant liquid separating the L-Gln from the strongly basic anion exchange resin was 8.0, the concentration of the eluant hydrochloric acid was 0.05 mol / L, and the elution rate was 1 .15%. The extraction yield of L-Gln under the above conditions was about 30%.