论文部分内容阅读
目的比较不同方法建立的衰老大鼠模型大脑皮层细胞凋亡与线粒体膜电位的变化状况。方法实验分为正常对照组、单纯腹腔注射D-半乳糖组、单纯摘除胸腺组、D-半乳糖注射联合胸腺摘除组、维生素E、C治疗组。造模及投药6w后,检测血清超氧化物歧化酶(SOD)水平和血清总抗氧化能力(T-AOC),并采用流式细胞术观察大脑皮层神经元细胞凋亡发生率以及神经元线粒体膜电位。结果造模及投药6w后,与对照组比较,3个造模组血清SOD水平和T-AOC均明显下降(P<0.05),造模3组之间无显著差异;神经元细胞凋亡发生率均明显上升,神经细胞线粒体膜电位均显著降低(P<0.05),以D-半乳糖腹腔注射联合胸腺摘除组尤为明显,与单一方法造模的两组比较该两项指标变化均有显著意义(P<0.05)。结论应用胸腺摘除复合D-半乳糖腹腔注射方法建立的衰老大鼠模型,更能体现出神经细胞衰老损伤的病理特征。
Objective To compare the changes of apoptosis and mitochondrial membrane potential in the cerebral cortex of aging rats established by different methods. Methods The experiment was divided into normal control group, simple intraperitoneal injection of D-galactose group, simple thymus group, D-galactose injection combined with thymus removal group, vitamin E, C treatment group. The levels of serum superoxide dismutase (SOD) and total anti-oxidative capacity (T-AOC) were measured after 6 weeks and 6 weeks of administration. The apoptotic rate of neurons in cerebral cortex and the percentage of mitochondria Membrane potential. Results Compared with the control group, the levels of serum SOD and T-AOC were significantly decreased (P <0.05), and there was no significant difference between the three groups in modeling and the apoptosis of neurons (P <0.05). The intraperitoneal injection of D-galactose combined with thymus removal group was more obvious. Compared with the single method of modeling, the changes of the two indexes were significant Significance (P <0.05). Conclusion The aging rat model established by intraperitoneal injection of D-galactose combined with thymus can better reflect the pathological characteristics of neuronal aging.