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为了进一步研究高原训练对大鼠红细胞生成的影响，本研究以大鼠１８Ｓ－ｒＲＮＡ为内参照，用定量反转录聚合酶链式反应（ＱＲＴ－ＰＣＲ），测定了６０只ＳＤ雄性大鼠在模拟不同海拔游泳训练后ＥＰＯ ｍＲＮＡ的变化。结果表明，所有高原对照组中ＥＰＯ ｍＲＮＡ水平均高于平原对照组，其中高原３０００米组和４０００米组较平原对照组有显著性增加Ｐ＜０．０５。在高原训练组中，除了高原２０００米训练后１周组及２周组较高原２０００米对照组ＥＰＯ ｍＲＮＡ水平有较大幅度增加外，其余各高原训练组ＥＰＯ ｍＲＮＡ水平较该组对照均有不同程度的下降。其中高原３０００米训练后１周组及２周组较高原３０００米对照组ＥＰＯ ｍＲＮＡ水平有显著水平的下降Ｐ＜０．０５，而高原４０００米训练后１周组及２周组较高原４０００米对照组ＥＰＯ ｍＲＮＡ水平有非显著水平的下降Ｐ＞０．０５。
In order to further study the effect of plateau training on erythropoiesis in rats, 18S-rRNA was used as an internal reference in this study. Quantitative reverse transcription-polymerase chain reaction (QRT-PCR) Simulating EPO mRNA changes after swimming training at different altitudes. The results showed that all the plateau control group EPO mRNA levels were higher than the plain control group, which plateau 3000 m and 4000 m group compared with the plain control group had a significant increase P <0.05. In the plateau training group, EPO mRNA levels in the plateau training group were significantly different from those in the control group except that the EPO mRNA levels in the 1-week group and the 2-week-old 2000-meter-long group increased significantly after the 2000-meter altitude training The degree of decline. The level of EPO mRNA decreased significantly at 1 week and 2 weeks after 3000 m training in plateau 3000 m compared with 3000 m in plateau, The level of EPO mRNA in the control group had a non-significant decrease (P> 0.05).