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目的:建立高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定消渴片中黄芪甲苷的含量。方法:色谱柱:Hypersil BDS C18色谱柱(4.6mm×200mm,5μm);流动相:乙腈-水(32∶68);ELSD漂移管温度:105℃;载气流速:2.7L·min-1;放大系数:1;撞击器:off。结果:黄芪甲苷在0.264～2.64μg范围内线性良好,回收率为98.36%,RSD为1.12%。结论:本法具有良好的精密度和重复性,结果准确可靠,可作为本产品的质量控制方法。
Objective: To establish a HPLC-ELSD method for the determination of astragaloside IV in Xiaoke Tablets. Method: Column: Hypersil BDS C18 column (4.6mm×200mm, 5μm); mobile phase: acetonitrile-water (32∶68); ELSD drift tube temperature: 105°C; carrier gas flow rate: 2.7L·min-1; Magnification factor: 1; impactor: off. RESULTS: The linearity of Astragaloside IV was good in the range of 0.264-2.64 μg. The recovery rate was 98.36% and the RSD was 1.12%. Conclusion: This method has good precision and repeatability, and the result is accurate and reliable. It can be used as a quality control method for this product.