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建立了分散液液微萃取-高效液相色谱法测定水样中氨苯磺胺、磺胺嘧啶,磺胺二甲基嘧啶3种磺胺药物残留的检测方法。对萃取剂、分散剂的种类和体积、pH、盐浓度等影响萃取效率的因素进行了优化。在最优的萃取条件下,3种抗菌药的测定线性范围为1~1000μg/L,r≥0.9997,检出限为0.07~0.25μg/L(S/N=3)。应用于5种不同水样中磺胺类抗菌药残留的分析,目标分析物的加标回收率在82.0%~104.0%之间,相对标准偏差小于5.9%。
A method for the determination of sulfanilamide, sulfadiazine and sulfamethazine residues in water samples by dispersive liquid-liquid microextraction and high performance liquid chromatography was established. Factors influencing extraction efficiency, such as extractant, type and volume of dispersant, pH and salt concentration were optimized. Under the optimal extraction conditions, the linear range of the three antibacterials was 1 ~ 1000μg / L, r≥0.9997, and the detection limit was 0.07 ~ 0.25μg / L (S / N = 3). It was applied to the analysis of sulfa antibacterial residues in five different water samples. The spiked recoveries of the target analytes ranged from 82.0% to 104.0% with relative standard deviations less than 5.9%.