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本实验应用自制心脏射频治疗仪,频率选择750KHz,输出形式为非调制正弦波形,连续输出,功率10～30W.仪器两极分别接左侧胸金属极板和USCI(6F或7F)普通电极导管.8只成年杂种犬静脉麻醉后气管插管.经皮股静脉穿刺法置入三根电极导管,分别位于高位右房、希氏束及右室尖部.术前常规电生理检查.以希氏束导管远端二极结合单极记录最大希氏束电位后,调整导管以保持较大的房室波(A/V)比例,于末端电极放电.一旦犬出现完全性房室传导阻滞(CAVB).再追加放电2次,以巩固效果.实验犬分别于急性期(0～14小时)或慢性期(3～4周)处死,除观察心脏大体所见外,其中6只犬采用连续切片方法进行了传导系统组织病理研究.
The experiment uses self-made cardiac radiofrequency treatment instrument, the frequency selection 750KHz, the output form is a non-modulated sine wave, continuous output, power 10 ~ 30 W. Instrument poles are connected to the left chest metal plate and USCI (6F or 7F) ordinary lead. Eight adult mongrel dogs were anesthetized by endotracheal intubation, and percutaneous femoral vein puncture was used to place three lead catheters in the upper right atrium, His bundle and right ventricle respectively.Preoperative preoperative electrophysiology was performed with His bundle Catheter distal bipolar junction monopolar record maximum His bundle potential, the catheter is adjusted to maintain a larger ratio of atrial (A / V) discharge at the terminal electrode Once the dog has a complete atrioventricular block (CAVB ) And then additional discharge 2 times in order to consolidate the effect of experimental dogs were killed in the acute phase (0 to 14 hours) or chronic phase (3 to 4 weeks), in addition to the general observation of the heart, of which 6 dogs with serial sections Methods Conducting histopathological studies.