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目的:研究复方芒果叶对博莱霉素诱导小鼠肺纤维化的作用。方法:建立博莱霉素诱导小鼠肺纤维化模型,观察复方芒果叶对模型动物整体行为学,HE、Masson染色后的组织病理学,以及检测肺指数、羟脯氨酸、丙二醛、还原型谷胱甘肽、超氧化物歧化酶、总抗氧化能力、炎症因子IL-1β、IL-6、TNF-α的表达水平。结果:复方芒果叶18.0 g/kg、9.0 g/kg及4.5 g/kg剂量组均可显著性降低肺泡炎和纤维化程度,降低肺指数以及羟脯氨酸、丙二醛、IL-6、TNF-α含量,同时,显著性升高超氧化物歧化酶含量和总抗氧化能力,复方芒果叶18.0 g/kg和9.0 g/kg剂量组均可明显改善肺纤维化小鼠整体行为学,升高还原型谷胱甘肽含量,复方芒果叶18.0 g/kg和4.5 g/kg剂量组均可显著性降低IL-1β含量。结论:复方芒果叶可减轻博莱霉素诱导肺纤维化小鼠的纤维化程度,降低其炎症和氧化水平,具有良好的抗炎、抗氧化、抗纤维化作用。
Objective: To study the effect of compound mango leaf on bleomycin-induced pulmonary fibrosis in mice. Methods: The model of pulmonary fibrosis induced by bleomycin was established in mice. The overall behavior of compound mango leaves was observed. The histopathology of HE and Masson staining, lung index, hydroxyproline, malondialdehyde, Reduced glutathione, superoxide dismutase, total antioxidant capacity, inflammatory cytokines IL-1β, IL-6 and TNF-α. Results: Compound mango leaves at doses of 18.0 g / kg, 9.0 g / kg and 4.5 g / kg significantly decreased alveolitis and fibrosis, decreased pulmonary index, hydroxyproline, malondialdehyde, IL-6, TNF-α, at the same time, significantly increased superoxide dismutase content and total antioxidant capacity, compound mango leaves 18.0 g / kg and 9.0 g / kg dose group can significantly improve the overall behavior of pulmonary fibrosis in mice, l Highly reduced glutathione content, compound mango leaves 18.0 g / kg and 4.5 g / kg dose group can significantly reduce IL-1β content. Conclusion: Compound mango leaves can reduce bleomycin-induced pulmonary fibrosis in mice with fibrosis, reduce their inflammation and oxidation levels, with good anti-inflammatory, anti-oxidation, anti-fibrosis.