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目的:建立一种操作简单、可重复、稳定的完全急性脊髓损伤(ASCI)动物模型,并对动物神经功能恢复进行初步评价。方法:将27只雌性小鼠随机分为3组(A/B/C),每组9只,钳夹法构建小鼠T9-T11节段ASCI模型,A组为钳夹2秒,B组为钳夹5秒,C组为钳夹10秒,采用改良组织学与行为学方法对模型动物脊髓损伤后神经功能恢复进行评价。结果:小鼠胸椎T9-T1节段急性钳夹损伤后,损伤脊髓处出现明显的出血瘢痕,后组织水肿,髓鞘变性,损伤处上下游出现空泡继而形成瘢痕等一系列的病理变化,3组比较在术后后肢运动功能BMS评分上有统计学差异(p<0.05)。3组术后组织学评分在脊髓损伤程度上有统计学差异(p<0.05)。结论:成功建立稳定性好、重复性强且具有治疗意义的小鼠脊髓钳夹损伤模型;(钳夹5秒组)具备较好的实验价值。
OBJECTIVE: To establish an animal model of complete acute spinal cord injury (ASCI) with simple, reproducible and stable operation and to evaluate the neurological function recovery of the animals. Methods: Twenty-seven female mice were randomly divided into 3 groups (A / B / C) with 9 rats in each group. The ASCI model of T9-T11 was constructed by clamping. 5 seconds for the clamp, C group for 10 seconds, improved histological and behavioral methods to evaluate the recovery of neurological function after model animals spinal cord injury. Results: After acute injury of T9-T1 segment of thoracic spine, there were obvious hemorrhagic scar in the injured spinal cord, tissue edema, demyelination, vacuolization on the upstream and downstream of the lesion and the formation of a series of pathological changes such as scars. There were significant differences in BMS scores of hindlimb motor function between the three groups (p <0.05). The postoperative histological scores of the three groups showed statistically significant differences (p <0.05) in the degree of spinal cord injury. CONCLUSION: Successful establishment of spinal cord clamp injury model with good stability, repetitiveness and therapeutic significance (clamping 5 seconds group) has good experimental value.