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目的研究Survivin基因在儿童急性白血病细胞中的表达,Survivin反义寡核苷酸对儿童急性白血病细胞凋亡的影响,并探讨反义寡核苷酸与传统化疗药物对白血病的协同治疗作用。方法用PCR的方法检查儿童白血病细胞Sur-vivin表达,将Survivin基因反义寡核苷酸转染入白血病细胞,用TUNEL及流式细胞仪检查各组细胞的凋亡率。结果Sur-vivin基因在儿童急性白血病细胞中高表达,Survivin反义寡核苷酸及足叶乙甙均能够单独诱导白血病细胞凋亡,两者合用时细胞凋亡率更高。结论Survivin在儿童急性白血病细胞中高表达;Survivin反义寡核苷酸可诱导白血病细胞凋亡;Sur-vivin反义寡核苷酸可促进足叶乙甙诱导的儿童急性白血病细胞凋亡。
Objective To investigate the expression of Survivin gene in childhood acute leukemia cells and the effect of Survivin antisense oligonucleotide on the apoptosis of childhood acute leukemia cells and to explore the synergistic therapeutic effect of antisense oligonucleotide and traditional chemotherapy drugs on leukemia. Methods The expression of Sur-vivin in childhood leukemia cells was detected by PCR. Antisense oligonucleotides of Survivin gene were transfected into leukemia cells. The apoptosis rate of each group was examined by TUNEL and flow cytometry. Results Sur-vivin gene was highly expressed in childhood acute leukemia cells. Both Survivin antisense oligonucleotide and etoposide could induce the apoptosis of leukemia cells alone. Conclusion Survivin is overexpressed in childhood acute leukemia cells. Survivin antisense oligonucleotide can induce apoptosis in leukemia cells. Survivin antisense oligonucleotide can promote etoposide-induced apoptosis in childhood acute leukemia cells.