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目的:对一起两种血清型副溶血性弧菌引起的食物中毒病原菌进行毒力基因及分子分型研究。方法:参照WS271-2007等标准对10名病人的肛拭进行致病菌的检测,对分离的副溶血性弧菌进行血清学试验;运用荧光定量PCR检测分离菌株的毒力基因,并用脉冲场凝胶电泳(PFGE)进行分子分型。结果:10份样本均检出O4:K8血清型副溶血性弧菌,其中5份样本同时检出O3:K6血清型副溶血性弧菌。两种血清型的副溶血性弧菌毒力基因检测均为tdh阳性、trh阴性。PFGE分型显示,同一血清型的菌株为高度相似克隆,相似度在92.9%～100%之间,同一样本不同血清型的菌株为不同克隆。结论:这是一起由O4:K8和O3:K6两种血清型副溶血性弧菌混合感染引起的食物中毒。
Objective: To study the virulence genes and molecular typing of food poisoning pathogens caused by two serotypes of Vibrio parahaemolyticus. Methods: According to the standard of WS271-2007 and so on, the pathogenic bacteria of anal swab in 10 patients were tested and the serotypes of Vibrio parahaemolyticus were tested. The virulence genes of isolates were detected by fluorescence quantitative PCR, Gel electrophoresis (PFGE) for molecular typing. Results: O4: K8 serotype Vibrio parahaemolyticus was detected in 10 samples, of which 5 samples were simultaneously detected O3: K6 serotype Vibrio parahaemolyticus. Vibrio parahaemolyticus virulence genes of the two serotypes were detected tdh positive, trh negative. PFGE typing showed that the strains of the same serotype were highly similar clones with similarity between 92.9% and 100%, and strains of different serotypes in the same sample were different clones. Conclusion: This is a food poisoning caused by a mixed infection of two serotype Vibrio parahaemolyticus O4: K8 and O3: K6.