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目的:通过观察抗感染喷剂对制备SD大鼠背部全层皮肤切除模型后创伤修复中血小板源性生长因子(PDGF)表达的影响,旨在从分子生物学角度探讨抗感染喷剂对创伤修复作用机制。方法:将健康SD大鼠随机分为抗感染喷剂实验组、康复新液药物对照组及生理盐水空白组,采用全层皮肤切除模型,于伤后12小时后分组外喷抗感染喷剂、康复新液、生理盐水,5ml/次,每日2次,连续用7日,在用药7d后取出肉芽组织。对PDGF采用免疫组化法和原位杂交后,进行统计学处理。结果:(1)SD大鼠皮肤创伤肉芽组织阳性细胞计数与远离创伤切口的皮肤相同结构处比较差异有统计学意义(P<0.01);(2)抗感染喷剂实验组与康复新液药物对照组比较,阳性细胞光密度及细胞阳性染色均有显著增多(P>0.05);(3)抗感染喷剂实验组、康复新液药物对照组分别与生理盐水空白组比较,阳性细胞光密度及细胞阳性染色均有显著增多(P<0.01)。结论:抗感染喷剂对SD大鼠皮肤创伤修复中PDGF阳性细胞的含量有显著促进作用。
OBJECTIVE: To observe the effect of anti-infective spray on the expression of platelet-derived growth factor (PDGF) in wound repair after SD rat’s dorsal full-thickness skin excision model, aiming to explore anti-infectious spray on wound healing from the perspective of molecular biology Mechanism. METHODS: Healthy Sprague-Dawley rats were randomly divided into anti-infective spray test group, Kangfuxin liquid drug control group and physiological saline blank group. The full-thickness skin excision model was used. After 12 hours, the wound was injected with anti-infective spray, Rehabilitation new solution, physiological saline, 5ml / time, 2 times a day, continuous use for 7 days, after taking the medicine 7d after the removal of granulation tissue. After PDGF was subjected to immunohistochemistry and in situ hybridization, statistical analysis was performed. Results: (1) There were significant differences in the number of positive cell counts of granulation tissue from skin wounds of SD rats and those of the same structure away from the skin of wounds (P<0.01); (2) Anti-infective spray test group and Kangfuxin liquid drug Compared with the control group, the positive cell density and positive staining of cells were significantly increased (P>0.05); (3) The optical density of positive cells was higher in the anti-infective spray test group and the Kangxinxin drug control group than in the saline blank group, respectively. Positive staining of cells and cells were significantly increased (P <0.01). Conclusion: The anti-infectious spray has significant promoting effect on the content of PDGF positive cells in SD rat skin wound repair.