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目的探讨还原型谷胱甘肽(GSH)对60Coγ射线引起的人肺腺癌A549细胞中p65蛋白表达情况的影响。方法按处理方法不同将人肺腺癌A549细胞分为假照组、单纯照射组、GSH组、GSH+照射组。免疫印迹法检测各组细胞质和细胞核中p65蛋白的表达情况;免疫荧光法检测各组细胞中p65在细胞质和细胞核中的定位变化。结果 60Coγ射线照射后,p65蛋白在细胞核中的表达量随着吸收剂量的增加逐渐增多;GSH+照射组与单纯照射组相比,细胞核中p65的表达明显减少;但p65在细胞总蛋白中的表达没有明显变化。免疫荧光法检测到单纯照射组与假照组比较,细胞核内p65的表达量增多;GSH+照射组细胞核内p65的表达量相对单纯照射组明显减少。结论γ射线照射后人肺腺癌A549细胞核内p65的表达量增多,但GSH可以减少p65在细胞核内的表达,抑制p65的入核。
Objective To investigate the effect of reduced glutathione (GSH) on the expression of p65 protein in human lung adenocarcinoma A549 cells induced by 60Coγ ray. Methods According to different treatment methods, human lung adenocarcinoma A549 cells were divided into sham group, irradiation group, GSH group and GSH + irradiation group. The expression of p65 protein in cytoplasm and nucleus of each group was detected by Western blotting. The localization of p65 in cytoplasm and nucleus of cells was detected by immunofluorescence. Results The expression of p65 protein in the nucleus gradually increased with the increase of absorbed dose after 60Co γ -ray irradiation. The expression of p65 in the nucleus was significantly decreased in GSH + irradiation group compared with the irradiation alone group. However, the expression of p65 protein in the total cell protein No significant change. Immunofluorescence assay showed that the expression of p65 in the nucleus was increased compared with the sham irradiation group. The expression of p65 in the nucleus of GSH + irradiation group was significantly decreased compared with the pure irradiation group. Conclusion The expression of p65 in human lung adenocarcinoma A549 cells increases after γ-ray irradiation, but GSH can reduce the expression of p65 in the nucleus and inhibit the entry of p65 into the nucleus.