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目的通过流式细胞仪对涎腺肿瘤DNA倍体、DI指数及S期细胞增生率的检测,分析这些参数与肿瘤组织学分级和淋巴结转移之间的关系,以探讨其对涎腺肿瘤的诊断和预后评估能力。方法对156例涎腺良性肿瘤、临界瘤及恶性肿瘤新鲜组织进行流式细胞仪分析。结果良性肿瘤组未检出异倍体;临界瘤组44例检出异倍体,S期增生率达最高,提示有恶变倾向;恶性肿瘤组异倍体的检出率最高。DI与淋巴结转移无关,与肿瘤组织学分级有关(P<0.01)。SPF与淋巴结转移有关(P<0.01)。结论DNA异倍体的检出是恶性肿瘤的重要特征之一;S期细胞增生率为恶性肿瘤增生的指标。临界瘤中检出异倍体预示有癌变倾向;SPF高揭示肿瘤增生活性强。DI可以鉴别细胞的良恶性;DI和SPF可以作为肿瘤细胞恶性程度及预后的指标。
Objective To detect the DNA ploidy, DI index and cell proliferation rate of salivary gland tumor by flow cytometry, and to analyze the relationship between these parameters and the histological grade and lymph node metastasis of salivary gland so as to investigate the diagnosis of salivary gland tumor And prognostic ability. Methods 156 cases of salivary gland benign tumor, borderline tumor and malignant tumor fresh tissue were analyzed by flow cytometry. Results There was no aneuploidy in the benign tumor group, 44 aneuploidy in the critical tumor group, and the highest rate of S-phase hyperplasia in the benign tumor group, which indicated the tendency of malignant transformation. The detection rate of aneuploidy in malignant tumor group was the highest. DI has nothing to do with lymph node metastasis, and histological grade (P <0.01). SPF was associated with lymph node metastasis (P <0.01). Conclusion Detection of aneuploid DNA is one of the most important features of malignant tumors. The rate of cell proliferation in S phase is an indicator of malignant tumor proliferation. Detection of aneuploidy in the borderline tumors indicates a predisposition to cancer; SPF reveals a strong tumor hyperplasia. DI can identify the malignancy of the cell; DI and SPF can be used as an indicator of the degree of malignancy and prognosis of the tumor cells.