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目的：为探讨新生儿感染性疾病与细胞因子的关系。方法：采用ＥＬＩＳＡ双抗夹心法对２４例新生儿感染性疾病急性期和２０例恢复期患儿外周血单个核细胞培养上清液中白细胞介素６（ＩＬ６）、白细胞介素８（ＩＬ８）、肿瘤坏死因子（ＴＮＦα）进行测定，并与２０例正常新生儿比较。结果：新生儿感染急性期各细胞因子水平较对照组明显升高，恢复期降至正常水平，在４例死亡病例中ＩＬ８、ＴＮＦα水平明显高于非死亡病例。结论：细胞因子参与了炎症反应过程，并可作为感染性疾病的一项重要检测指标；ＩＬ８、ＴＮＦα水平的高低可反映疾病的严重程度，对指导临床转归具有一定价值。
Objective: To investigate the relationship between neonatal infectious diseases and cytokines. Methods: ELISA double-antibody sandwich method in 24 cases of neonatal infectious disease in acute and 20 cases of recovery of peripheral blood mononuclear cell culture supernatant of interleukin-6 (IL 6), interleukin 8 (IL 8), tumor necrosis factor (TNF α) were measured and compared with 20 normal newborns. Results: The levels of cytokines in acute stage of neonatal infection were significantly higher than those in control group, and the recovery period was reduced to normal level. The levels of IL-8 and TNF-α in 4 death cases were significantly higher than those in non-death cases. Conclusion: Cytokines are involved in the process of inflammatory reaction and can be used as an important detection index of infectious diseases. The level of IL-8 and TNF-α can reflect the severity of the disease and has certain value in guiding the clinical outcome.