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目的 为研究胰淀素（ Ａｍｙｌｉｎ） 对大鼠胰岛细胞分泌胰岛素的影响和机理。方法 用放免和荧光方法分别检测经不同浓度胰淀素温育的大鼠胰岛细胞的胰岛素分泌量、细胞内 Ｃａ 《及ｃ Ａ Ｍ Ｐ 含量。结果 在胰淀素１０μｍｏｌ／ Ｌ 组中高糖刺激下的胰岛素含量、细胞内 Ｃａ 《含量，细胞内ｃ Ａ Ｍ Ｐ 含量分别为０ ．８９ ±０ ．２１ｎｇ／ ｍｌ、６３ ±１０ｎｍｏｌ／ Ｌ、２９ ．３ ±３ ．３ｐｍｏｌ／１ ×１０６ 细胞与对照组（１ ．７５ ±０ ．４２ｎｇ／ｍｌ、１３５ ±１０ｎ ｍｏｌ／ Ｌ、３６ ．３ ±７ ．４ｐｍｏｌ／ Ｌ） 比较，其中胰岛素分泌量、细胞内 Ｃａ 《含量均明显降低， Ｐ＜ ０ ．０１ ，细胞内ｃ Ａ Ｍ Ｐ 含量有减少趋势。结论 高浓度的胰淀素抑制了胰岛细胞内 Ｃａ 《升高和／ 或细胞内ｃ Ａ Ｍ Ｐ 形成，可能是胰岛素分泌减少的原因之一。
Objective To study the effect and mechanism of Amylin on insulin secretion in pancreatic islets of rats. Methods The insulin secretion, intracellular Ca (superscript 2 +) and cA M P contents in pancreatic islet cells incubated with different concentrations of amylin were detected by radioimmunoassay and fluorescence. Results In the amylin 10 μmol / L group, the contents of insulin, intracellular calcium and intracellular c A M P were 0 under high glucose stimulation respectively. 89 ± 0. 21 ng / ml, 63 ± 10 nmol / L, 29. 3 ± 3. 3 pmol / 1 × 106 cells compared with the control group (1.75 ± 0.42ng / ml, 135 ± 10nmol / L, 36.3 ± 7.4 mol / L), in which insulin secretion, intracellular Ca Significantly lower, P <0. 01, intracellular c A M P content has a decreasing trend. Conclusions High concentration of amylin can inhibit the increase of intracellular Ca content and / or the formation of intracellular c A M P in pancreatic islets, which may be one of the reasons for the decrease of insulin secretion.