论文部分内容阅读
从人食管癌、胃癌、直肠癌、肝癌及胎儿肝和从距癌>5cm的癌旁作为对照组织的细胞核中,分别用0.4MNaCl盐提法、苯酚提取法和羟基磷灰石柱层析法提取染色质非组蛋白蛋白质,简称非组蛋白(NHP)进行电泳图谱分析并将不同方法提取的癌NHP作为抗原制备免疫血清做对比免疫组化反应。结果表明①用0.4MNaCl盐提之NHP的质和量更适于作为抗原制备免疫血清和进行免疫组化染色。②以上各种肿瘤NHP与其癌旁NHP之间均有质和量的差异。③不同肿瘤NHP具有组织特异性,然而各种瘤之间也具有相似的共性,即癌NHP之多加带主位于6万d～9万d;多变带区主位于4万d上下处(肝癌NHP之多变带区主位于5万d及8万d);而且肝癌NHP与胎肝NHP有相似处。结果提示癌NHP之多加或加深带可能与癌基因表达的激活有关;而缺如或减弱带可能与载有抗癌基因的染色体区带丢失或基因表达改变有关。
From the esophageal cancer, gastric cancer, rectal cancer, liver cancer and fetal liver, and the nucleus as a control tissue from a tumor >5 cm away from the cancer, 0.4 M NaCl salt extraction, phenol extraction, and hydroxyapatite column chromatography, respectively Chromatin non-histone proteins were extracted, and the non-histone protein (NHP) was used for electrophoretogram analysis. The immunohistochemical reactions were prepared by immunohistochemistry using antigen extracted from cancerous NHP extracted by different methods. The results showed that the quality and quantity of NHP extracted with 0.4 M NaCl salt were more suitable for the preparation of immune serum as an antigen and immunohistochemical staining. More than 2 kinds of tumors NHP and its adjacent NHP have qualitative and quantitative differences. 3 Different tumors NHP has tissue-specificity, but all tumors share similarities in common, that is, the majority of cancer NHP is mainly located in 60,000-90,000 days; the main area of mutation is located in the upper and lower parts of 40,000 days (liver cancer The NHP’s multivariable region is mainly located at 50,000 d and 80,000 d); and there is a similarity between NHP and fetal liver NHP. The results suggest that the increased or darkened bands of NHP may be related to the activation of oncogene expression, while the absence or attenuation bands may be related to the loss of chromosomal regions containing anti-oncogenes or altered gene expression.