论文部分内容阅读
目的探讨X射线交错互补修复基因3(XRCC3)Thr241Met和人类着色性干皮病基因D(XPD)Asp312Asn及Lys751Gln与职业性慢性铅中毒的关系。方法选择103名蓄电池厂确诊为职业性慢性铅中毒的男性工人为病例组,按照工种和年龄1∶1匹配选择同厂无诊断为铅中毒的铅作业男性工人为对照组。采集2组人群周围静脉血,采用聚合酶链反应-限制性片段长度多态性技术检测XRCC3 Thr241Met、XPD Asp312Asn和XPD Lys751Gln位点的多态性,采用条件Logistic回归分析分析不同基因型与职业性慢性铅中毒的关系。结果 2组人群中XRCC3Thr241Met、XPD Asp312Asn和XPD Lys751Gln多态位点的基因型分布均符合遗传学Hardy-Weinberg平衡定律(P>0.05)。2组人群XRCC3 Thr241Met 3种基因型总体分布比较,差异有统计学意义(P<0.05)。2组人群XPD Asp312Asn和XPD Lys751Gln 3种基因型总体分布分别比较,差异均无统计学意义(P>0.05)。XRCC3 Thr241Met的CT和TT基因型均增加铅中毒易感性[校正比值比分别为2.44和3.37,95%可信区间为(1.17~5.09)和(1.09~11.14)],而XPD Asp312Asn和XPD Lys751Gln不同基因型与铅中毒易感性无关(P>0.05)。结论 XRCC3Thr241Met的CT或TT基因型与职业性慢性铅中毒相关;而XPD Asp312Asn和XPD Lys751Gln多态性与职业性慢性铅中毒易感性无关。
Objective To investigate the relationship between XRCC3 Thr241Met and human chromosomal dry skin disease gene D (XPD) Asp312Asn and Lys751Gln and occupational chronic lead poisoning. Methods A total of 103 male workers diagnosed as occupational chronic lead poisoning in the battery factory were selected as the case group. According to the 1: 1 type of work-age and the age, lead workers with no diagnosis of lead poisoning were selected as the control group. The peripheral venous blood was collected from two groups of people and the polymorphisms of XRCC3 Thr241Met, XPD Asp312Asn and XPD Lys751Gln were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Logistic regression analysis was used to analyze the genotype and occupational Relationship between chronic lead poisoning. Results The genotype distributions of XRCC3Thr241Met, XPD Asp312Asn and XPD Lys751Gln polymorphic loci in both groups were in accordance with the Hardy-Weinberg equilibrium (P> 0.05). There were significant differences in the overall distribution of XRCC3 Thr241Met among the two groups (P <0.05). There were no significant differences in the overall distribution of XPD Asp312Asn and XPD Lys751Gln among the two groups (P> 0.05). The CT and TT genotypes of XRCC3 Thr241Met increased susceptibility to lead poisoning (adjusted odds ratios, 2.44 and 3.37, respectively, with 95% confidence intervals (1.17-5.09) and (1.09-11.14)], whereas XPD Asp312Asn and XPD Lys751Gln differed The genotype had no correlation with susceptibility to lead poisoning (P> 0.05). Conclusion The CT or TT genotype of XRCC3Thr241Met is associated with occupational chronic lead poisoning. However, the polymorphisms of XPD Asp312Asn and XPD Lys751Gln are not associated with the susceptibility to occupational chronic lead poisoning.