论文部分内容阅读
以采自芜湖市镜湖水体中的11个角突臂尾轮虫(Brachionus angularis)克隆为对象,在(25±1)℃,0.5×10~6、1.0×10~6和2.0×10~6个细胞/m L的斜生栅藻(Scenedemus obliquus)密度下,应用群体累积培养法研究了角突臂尾轮虫种群增长参数的克隆多样性.结果表明,轮虫的种群增长率、最大种群密度、种群中的平均混交率和平均受精率与食物密度之间的关系具有较高的克隆多样性,食物密度、克隆以及两者间的交互作用对轮虫种群增长率、最大种群密度、平均混交率和平均受精率均有极显著性影响(P<0.01).研究角突臂尾轮虫种群增长参数的克隆在所有的食物密度和克隆组合中,克隆2在2.0×10~6个细胞/m L食物密度下的种群增长率和最大种群密度均最高,克隆3在1.0×10~6个细胞/m L食物密度下平均混交率最高,克隆3和9在2.0×10~6个细胞/m L食物密度下的平均受精率最高.因此,在开展角突臂尾轮虫的规模化培养时,拟首选克隆2和2.0×10~6个细胞/m L的斜生栅藻密度;而欲开展角突臂尾轮虫休眠卵的批量生产,则拟选择克隆3和1.0×10~6个细胞/m L的斜生栅藻密度.
The Brachionus angularis clones collected from Jinghu Lake in Wuhu city were collected at temperatures of (25 ± 1) ℃, 0.5 × 10 ~ 6, 1.0 × 10 ~ 6 and 2.0 × 10 ~ At population density of 6 cell / m L Scenedesmus obliquus, population diversity was used to study the clonal diversity of growth parameters of Brachionus pomonella population. The results showed that the population growth rate of rotifers was the largest The population density, the average mixed rate in the population, the average fertilization rate and the food density had a higher clonal diversity, the density of the food, the cloning and the interaction between the population growth rate, the maximum population density, (P <0.01) .Study on clonal population growth parameters of Brachionus obliquus In all the food density and clonal combinations, clone 2 was found in 2.0 × 10 ~ 6 The population growth rate and maximum population density were the highest under the cell / m L food density. The average hybridization rate of clone 3 under the density of 1.0 × 10 ~ 6 cells / m L was the highest, while that of clone 3 and 9 was 2.0 × 10 ~ 6 The highest average fertilization rate was observed at cell / mL dietary density. Therefore, When modeling culture, we prefer clone 2 and 2.0 × 10 ~ 6 cells / m L of Lasiopodomysia density; To carry out mass production of dormant eggs of Brachionus pardus, clones 3 and 1.0 × Obliquasmus density of 10-6 cells / m L.