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目的 研究 FN6 - 8片段能否促进损伤神经元的突起生长。 方法 从包含有 TN- C分子中 FN6 -8DNA序列的质粒中表达并纯化 GST- FN6 - 8融合蛋白 ,以 0 .0 5 mg/ L 的浓度加入培养的胚胎小鼠脊髓神经元的培养液中 ,对照组加入等量 GST,然后液体石蜡封闭液面造成神经元缺气损伤 ,3d后做 MTT实验 ,测神经元活性并图像分析神经元突起的长度。 结果 FN6 - 8组的神经元活性和神经元突起长度明显高于对照组。 结论 TN- C促进神经元活性及突起生长的功能可能由其 FN6 - 8片段介导。
Objective To investigate whether FN6 - 8 could promote neurite outgrowth. Methods The GST - FN6 - 8 fusion protein was expressed and purified from the plasmid containing the FN6 - 8 DNA sequence in TN - C molecule and added to the culture medium of embryonic mouse spinal cord neurons at the concentration of 0. 05 mg / L , The same amount of GST was added into the control group, and then the liquid paraffin-blocked liquid level caused neuronal aeroallergenic injury. MTT assay was performed after 3d to measure the neuronal activity and analyze the length of neurite protrusion. Results The neuronal activity and neurite length in FN6 - 8 group were significantly higher than those in control group. Conclusion The function of TN-C in promoting neuronal activity and neurite outgrowth may be mediated by its FN6 - 8 fragment.