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目的对广东省深圳市同一餐馆连续发生的两起食物中毒事件进行流行病学调查并现场取样进行实验室应急检测,查找中毒原因。方法样品碱化后用乙酸乙酯提取,再用稀盐酸反萃取,萃取的样液pH调至5.2后用SCX固相萃取小柱净化,分离所得的盐酸克伦特罗经双三甲基硅烷三氟乙酰胺(BSTFA)衍生后用气相色谱电子轰击源质谱分析,选择离子监测(SIM)模式检测辅以全扫描(Scan)质谱图的NIST谱库检索匹配确认,美托洛尔内标法定量。结果在食物中毒现场采集的八步蛇肉、血、胆及病人尿液中均检出了盐酸克伦特罗,浓度分别为1331、463、11200、4.3μg/kg。后续采集的蛇样品中未再检出盐酸克伦特罗。结论两起食物中毒是由于食用了含有盐酸克伦特罗的蛇所致,这种因食用蛇中残留盐酸克伦特罗而引起食物中毒的事件,在国内外尚属首例。
Objective To carry out epidemiological investigation of two consecutive food poisoning incidents in the same restaurant in Shenzhen, Guangdong Province, and carry out emergency laboratory tests to find out the causes of poisoning. Methods The samples were alkalized, extracted with ethyl acetate, and then extracted with dilute hydrochloric acid. The pH of the sample was adjusted to 5.2 and purified by SCX solid phase extraction cartridge. The resulting clenbuterol bistrimethylsilane tri Fluoroacetamide (BSTFA) was derivatized with gas chromatographic electron impact mass spectrometry, selective ion monitoring (SIM) mode detection and NIST spectral library matching search with full scan mass spectrometry, metoprolol internal standard quantification . Results Clenbuterol hydrochloride was detected in eight steps of snake meat, blood, gall bladder and urine collected from patients on food poisoning. The concentrations were 1331, 463, 11200 and 4.3 μg / kg, respectively. Clenbuterol hydrochloride was no longer detected in subsequent snake samples. Conclusions The two food poisonings were caused by the consumption of snake containing clenbuterol, which is the first case of food poisoning caused by residual clenbuterol in snake.