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用放射免疫法（ＩＲＭＡ）测定血清促甲状腺激素，观察正常１６例，Ｇｒａｖｅｓ病治疗前１２例、治疗后６例和Ｇｒａｖｅｓ病２９例经治疗、临床上治愈后撤药患者的ＴＳＨ昼夜节律。同时观察ＴＲＨ兴奋实验。结果显示：正常人ＴＳＨ分泌具有昼夜节律特征；Ｇｒａｖｅｓ病治疗前后，ＴＳＨ昼夜节律消失；ＴＲＨ兴奋实验已恢复正常的撤药患者中，仍有１０例（３４．５％）ＴＳＨ昼夜节律未恢复正常。提示，ＴＳＨ昼夜节律测定是Ｇｒａｖｅｓ病早期诊断和判断治愈的有效指标之一。
Serum thyrotropin was determined by radioimmunoassay (IRMA). The results showed that 16 cases were normal, 12 cases were treated with Graves disease, 6 cases were treated with Graves’ disease and 29 cases were cured. At the same time observe the TRH excited experiment. The results showed that the circadian rhythm of TSH secretion was normal in normal people, the circadian rhythm of TSH disappeared before and after treatment of Graves disease, and the circadian rhythm of TSH did not return to normal in 10 (34.5%) patients whose TRH excitability test was normal. . Tip, TSH circadian rhythm determination of Graves disease diagnosis and early diagnosis and treatment to determine one of the effective indicators.