作为Ca~(2+)在细胞内的主要受体,钙调素(Calmodulin,CaM)广泛存在于各种真核细胞中,是一种能调节细胞生长、分化和转化的多功能蛋白。 由于CaM的反义RNA相对于它的多种拮抗剂来说具有更强的特异性并且没有药理毒性作用,因此我们构建了表达钙调素反义RNA的人肝癌细胞模型,并对此模型在钙调素低表达状态下的生长特性、周期分布、基因表达及其与转化的关系进行了分析,以探讨钙调素对细胞增殖与转化的影响。 As the main receptor for Ca~(2+) in cells, calmodulin (CaM) is widely present in various eukaryotic cells and is a multifunctional protein that can regulate cell growth, differentiation, and transformation. Since CaM antisense RNA is more specific than its antagonists and has no pharmacological effects, we have constructed a human liver cancer cell model expressing calmodulin antisense RNA, and The relationship between the growth characteristics, cycle distribution, gene expression, and the transformation of calmodulin under low expression status was analyzed to investigate the effects of calmodulin on cell proliferation and transformation.