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分析近年国内外单倍体育种研究理论与经验,经美国著名单倍体育种家张铭堂指导,组装集成出本技术规范,供试用参照。本规范制定了杂交诱导单倍体生殖方法选育自交系的基本操作程序,将单倍体杂交诱导、子粒鉴选、植株确认、自然与人工加倍及双单倍体获得5个环节作出技术规定。首次提出“诱导系杂交组合”、“二环双单系”的概念与应用效果。化学加倍环节是技术关键,属各公司商业机密范畴,未加细化,仅就要点说明。经多年实践验证,规模化应用该规范程序,只需2～3个生育世代即可批量选育出遗传高度纯合的玉米自交系(DH系)。
Analysis of recent years, the theory and experience of haploid breeding research both at home and abroad, under the guidance of Zhang Mingtang, a famous haploid breeder in the United States, assembled and integrated this technical specification for reference. This norm establishes the basic operation procedure of hybrid inducible haploid reproductive method to inbred inbred lines. It will make haploid hybridization induction, seed selection, plant confirmation, natural and artificial doubling and double haploid to obtain 5 links Provisions. For the first time, the concept and application effect of “Induction line hybrid combination ” and “Bicyclic Double Line ” were proposed. Doubling of chemical links is the key technology, which is a category of commercial confidentiality of companies, without refinement, only to point instructions. After many years of practice, large-scale application of the standard program, only 2 to 3 generations of breeding can be batch breeding of highly homozygous maize inbred lines (DH line).