目的 制备并研究bFGF单克隆抗体,以便用于恶性肿瘤的早期诊断。方法 提纯bFGF并用杂交瘤技术获得杂交瘤细胞,再用BALB/C鼠种植收获腹水得到McAb,进行核型、效价、特异性、表位等分析。结果 所得杂交瘤细胞具有双亲细胞染色体特点McAb效价较高,特异性良好,无非特异反应,3种McAb均为IgG型。结论 McAb具有良好的性能,为进一步的临床检测应用奠定了基础。 Objective To prepare and study bFGF monoclonal antibody for early diagnosis of malignancy. Methods The bFGF was purified and hybridomas were used to obtain hybridoma cells. Then BALB/C mice were used to harvest ascitic fluid to obtain McAbs. The karyotype, titer, specificity, and epitopes were analyzed. Results The obtained hybridoma cells had the chromosomal characteristics of the parental cells. The McAb had high titer, good specificity, and no non-specific reaction. All three McAbs were of IgG type. Conclusion The McAb has good performance and lays a foundation for further clinical detection applications.