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以陆地棉岱字-15号棉纤维细胞为材料,用~3H-葡聚糖示踪方法测定β-1,3-葡聚糖和纤维素的合成。PEG4000促进分β-1,3-葡聚糖和纤维素的合成,对刺激纤维素的合成更有效;随着非离子型表面活性剂Trion X-100和Tween20浓度的升高,抑制β-1,3-葡聚糖和纤维素的合成程度也增加,但抑制纤维素的合成更为强烈;而阴离子表面活性剂SDS则有所不同,在较高浓度下,又出现对β-1,3-葡聚糖合成抑制的减弱,这可能与SDS载负电荷的缘故有关。结果提示,完整的细胞膜有利于纤维素的合成,细胞膜损伤则利于β-1,3-葡聚糖的合成。
The synthesis of β-1,3-glucan and cellulose was determined by the ~ 3H-dextran method using the land cotton Dai-15 cotton fiber cells as materials. PEG4000 promoted the synthesis of β-1,3-glucan and cellulose, and was more effective in stimulating the synthesis of cellulose. With the increase of the concentration of nonionic surfactant Trion X-100 and Tween20, the inhibition of β-1 , 3-glucan and cellulose synthesis also increased, but the inhibition of cellulose synthesis is more intense; anionic surfactant SDS is different at higher concentrations, appeared again β-1,3 - The decrease in dextran synthesis inhibition may be related to SDS negative charge. The results suggest that the intact cell membrane is conducive to the synthesis of cellulose, cell membrane damage is conducive to the synthesis of β-1,3-glucan.