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目的:建立满山红提取物中金丝桃苷、杜鹃素、槲皮素和山奈酚总量的高效液相色谱测定方法。方法:采用高效液相色谱法测定满山红提取物中4种黄酮类成分的总量。结果:金丝桃苷、杜鹃素、槲皮素、山奈酚的线性范围分别为19.248~481.2μg·ml-1(r=0.9999)、7.984~199.6μg·ml-1(r=0.9998)、5.648~141.2μg·ml-1(r=0.9996)、1.408~35.2μg·ml-1(r=0.9998)、平均加样回收率分别为96.5%、98.4%、97.4%、97.5%,RSD分别为1.25%、0.70%、0.71%、0.89%。四批满山红提取物中多个黄酮成分总量分别为3.17%、3.14%、3.06%、3.19%。结论:该方法简便、重复性好、结果准确可靠,可用于满山红提取物的质量控制。
Objective: To establish a HPLC method for the determination of the total amount of hyperoside, farrellin, quercetin and kaempferol in the extract of Momoyama red. Methods: The total amount of four flavonoids in the extract of Momordica cusia was determined by high performance liquid chromatography. Results: The linear ranges of hyperin, farrerol, quercetin and kaempferol were 19.248 ~ 481.2μg · ml-1 (r = 0.9999), 7.984-199.6μg · ml-1 (r = 0.9998) (R = 0.9996) and 1.408 ~ 35.2μg · ml-1 (r = 0.9998) respectively. The average recoveries were 96.5%, 98.4%, 97.4% and 97.5%, respectively %, 0.70%, 0.71%, 0.89%. The total flavonoids in the four batches of Manshanhong extract were 3.17%, 3.14%, 3.06% and 3.19% respectively. Conclusion: The method is simple, reproducible, accurate and reliable, and can be used for the quality control of Mangosteen extract.