论文部分内容阅读
建立了分离逆转录聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ）产物的高效液相色谱方法，反应液直接进样， 用 Ｔ Ｓ Ｋ ｇｅｌ Ｄ Ｅ Ａ Ｅ Ｎ Ｐ Ｒ 柱分离， Ｔｒｉｓ Ｈ Ｃｌ 缓冲溶液（ｐ Ｈ ９０）氯化钠线性梯度洗脱，于 ２６０ ｎｍ 处检测。用所建立的方法分析了大鼠肠缺血／再灌注损伤后外周血中性粒细胞（ Ｐ Ｍ Ｎ）磷脂酶 Ａ２ ｍ Ｒ Ｎ Ａ 的表达。
A high performance liquid chromatography (HPLC) method was established for the separation of RT-PCR products. The reaction solution was directly injected into the column and separated on a TSK gel D E A N-N P R column. Tris-H Cl buffer solution (p H 9 0) sodium chloride gradient elution, detected at 260 nm. The established method was used to analyze the expression of phospholipase A2 m R N A in peripheral blood after intestinal ischemia / reperfusion injury in rats.